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The 44th Annual Gainesville Cycling Festival takes place March 28-29, 2026, organized by Gainesville Cycling Club. Saturday features the Seacoast Bank Santa Fe Century and Gravel Challenge starting at Daft Cow Brewery, showcasing scenic rural roads north of Gainesville. Sunday offers the Horse Farm Hundred and Horse Farm Tours starting at First Magnitude Brewing Company, heading south through Marion County’s picturesque horse country with significant elevation gain. Registration is $60-$90 depending on timing and membership status ($45-$75 for GCC members). The event includes rest stops, SAG support, and various distance options. Proceeds benefit Boys & Girls Club of Northeast Florida and the club’s Bikes 4 Vets program.